Simmer, don’t cycle—a molecular biologist’s guide to RPA

Monika Jazdauskaitė, R&D scientist at Thermo Fisher Scientific, explains the fundamentals of Recombinase Polymerase Amplification (RPA) and how this isothermal technique is transforming both target detection and NGS workflows. From pathogen identification to sequencing library prep, RPA offers speed, simplicity, and flexibility without even thermal cycling.